pMEV2HA-MEK1-DN

Human MEK1 Xpress Clone, dominant negative MEK1 and MEK2 are members of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. MEKs are involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.Several residues are essential for MEK to function: K97 for its catalytic activity, S218 and S222 for activation by upstream activators. The dominant negative mutant provided (P1030b) contains 3 point mutations (K97R; S218A; S222A) and can neither be phosphorylated by its activators nor phosphorylate its downstream effectors (ERKs). The constitutively active mutant (P1030c) contains 2 point mutations (S218E and S222E) and a deletion of amino acid residues 31-52. The resulting protein is ~400 times more active than recombinant MEK1 wild type expressed in E. coli, and is active without the need of being phosphorylated by its upstream activators. - 20 ug